Regulation of T Cell Responses by Nano-Hydroxyapatite to Mediate the Osteogenesis

Regulation of T Cell Responses by Nano-Hydroxyapatite to Mediate the Osteogenesis

Blog Article

Nano-hydroxyapatite (nHA) has been widely applied as a tissue-engineering biomaterial and interacted with osteoblasts/stem cells to repair bone defects.In addition, T cells that coexist with osteoblasts/stem cells in the bone modulate the regulation of osteoimmunology by cytokine formation.However, the effects of nHA on T cells and the following regulatory interplay on osteogenic differentiation have been rarely examined.In this work, the physicochemical properties of needle-like nHA are characterized by field emission scanning electron microscopy, zeta potential, Fourier transform-infrared ultrastar dc hc550 and X-ray diffraction.

It is found that as the concentration of nHA increases, the proliferation of T cells gradually increases, and the proportion of apoptotic T cells decreases.The percentage of CD4+ T cells is higher than that of CD8+ T cells under the regulation of needle-like nHA.Furthermore, the supernatant of T cells co-cultured with nHA significantly inhibits the osteogenic differentiation of MC3T3-E1 by animed aniflex complete downregulating the formation of alkaline phosphatase and calcium nodule compared with the supernatant of nHA.Thus, our findings provide new insight into the nHA-mediated T cell and osteoblast interactions.